Search results for " DAS-ELISA"
showing 4 items of 4 documents
Genetic structure and molecular variability of grapevine fanleaf virus in sicily
2021
Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in four Sicilian provinces (Italy), and its genetic structure and molecular variability were studied. In detail, a total of 617 grapevine samples of 11 autochthonous grapevine cultivars were collected in 20 commercial vineyards. Preliminary screening by serological (DAS-ELISA) and molecular (RT-PCR) analyses for ArMV (arabis mosaic virus) and GFLV detection were conducted. Results obtained showed the absence of ArMV in all the s…
First report of grapevine fleck virus in vineyards of Bosnia and Herzegovina
2020
Grapevine is one of the most important fruit crops in Bosnia and Herzegovina with an annual production of around 40 thousand tons in 2018 (FAOSTAT, http://www.fao.org/ faostat/en/#data/QC). Production of grapevine in the country is mainly concentrated in vineyards in the Lištica and Mostar regions of Herzegovina with the two predominant autochthonous cultivars ‘Žilavka’ and ‘Blatina’. Grapevine fleck virus (GFkV) from the genus Maculavirus in the family Tymoviridae is a phloem limited virus with a positive-sense single-stranded RNA genome (Dreher et al. 2012). GFkV is associated with fleck disease of Vitis spp. In order to investigate the current status of GFkV in Bosnia and Herzegovina, 80…
Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability
2022
Background: the most widely distributed and virulent Grapevine leafroll-associated viruses (GLRaV) that affect grapevine are GLRaV-1 and GLRaV-3, transmitted semi-persistently by different mealybugs and soft scales, mainly causing downward rolling of the leaf margins and interveinal reddening. Methods: the main objectives of this study were to investigate the genetic structure and molecular diversity of GLRaV-1 and GLRaV-3 in 617 samples from 11 autochthonous Sicilian grapevine cultivars, ascertaining their presence and spread. The detection was implemented by serological and molecular analyses and subsequently phylogenetic analyses on selected Sicilian isolates were conducted. Results: in …
Evolutionary Analysis of Grapevine Virus A: Insights into the Dispersion in Sicily (Italy)
2022
Grapevine virus A (GVA) is a phloem-restricted virus (genus Vitivirus, family Betaflexiviridae) that cause crop losses of 5–22% in grapevine cultivars, transmitted by different species of pseudococcid mealybugs, the mealybug Heliococcus bohemicus, and by the scale insect Neopulvinaria innumerabilis. In this work, we studied the genetic structure and molecular variability of GVA, ascertaining its presence and spread in different commercial vineyards of four Sicilian provinces (Italy). In total, 11 autochthonous grapevine cultivars in 20 commercial Sicilian vineyards were investigated, for a total of 617 grapevine samples. Preliminary screening by serological (DAS-ELISA) analysis for GV…